Collagen-derived dipeptide prolyl-hydroxyproline promotes

differentiation of MC3T3-E1 osteoblastic cells

Yoshifumi Kimira ^a,*, Kana Ogura ^a, Yuri Taniuchi ^a, Aya Kataoka ^b, Naoki Inoue ^b, Fumihito Sugihara ^b, Sachie Nakatani ^a, Jun Shimizu ^a, Masahiro Wada ^a, Hiroshi Mano ^a 

a Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan

b Nitta Gelatin Inc., Peptide Division, 2-22 Futamata, Yao, Osaka 581-0024, Japan

Abstract

Prolyl-hydroxyproline (Pro-Hyp) is one of the major constituents of collagen-derived dipeptides. The objective of this study was to investigate the effects of Pro-Hyp on the proliferation and differentiation of MC3T3-E1 osteoblastic cells. Addition of Pro-Hyp did not affect MC3T3-E1 cell proliferation and matrix mineralization but alkaline phosphatase activity was significantly increased. Furthermore, cells treated with Pro-Hyp significantly upregulated gene expression of Runx2, Osterix, and Colla1. These results indicate that Pro-Hyp promotes osteoblast differentiation. This study demonstrates for the first time that Pro-Hyp has a positive effect on osteoblast differentiation with upregulation of Runx2, Osterix, and Colla1 gene expression.